First, we must have cells in order to test the effectiveness of drugs. So, here is the flask of cells where we culture them. MDA-MB-468 cells are our favourite kid because they grow up very healthily, fast & not so fussy. The most headache cells are HCC because up to this moment, we are still short of them (they grow extremely slow). Some of them even died prematurely.
Next, before using the cells, we must check under microscope.
Check what? The shape and its confluency. If anything goes wrong,
we need to grow them AGAIN.
Okay, if everything is fine. Proceed to the next step.
All the tasks are done in this biosafety cabinet which claims to be sterile.
Only hands are allowed inside the cabinet.
After all the long & fussy steps, here come my final step.
In which you will see a variety of purple colour. The more purplish it's, the more cells are present, i.e. your drug is INEFFECTIVE.
So, we usually aim for light purple. We want the cells DIE!
See a trend from deep purple to light? Yeah my drug works! Happy in term of this is a new finding, sad because of more works have to be done in order to further reassure it's effective :S
Dilemma.
Lastly, analyzing the result using ELISA plate reader.
Where now results will be converted into a lots of numbers.
So, that's my routine for the entire semester.
3 working days in a week. Not more than 5 hours per day.
Great isn't it? I doubt. Aimless life I'm having now.
Feel guilty without revising anything. How?
Just let it be for temporary HAHAHAHA
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